Researches concerning the daily deuterium depleted water intake correlated with the values of some clinical and paraclinical parameters in healthy and inoculated with Ehrlich ascite NMRI mice, страница 2

On the other hand, biochemical determinations revealed a significant decrease of total plasma proteins’ values compared to the reference group 1, the variation limits of which frame into the literature data [5]. The most obvious negative differences were obtained (fig.4) between groups 3 and 1 (-91,66%), and groups 4 and 1 (-32,99%).

To complet the value of the obtained data, the relative and absolute weight values of thymus, spleen, adrenal glands and lymph nodes were determined (fig. 5).  The most significant negative differences were obtained in the case of group 3, and consisted of decreased spleen and thymus weight, while in the case of group 4 the obtained data records positive differences compared to the reference group 1.


Concerning the relative and absolute lymph nodes’ weight, the most affected are the animals from the group 3 (negative values, both the axillary and the sacral lymph nodes), while in the case of group 4 the negative differences are not so expressive (fig. 6). This aspects demonstrate that the deuterium-depleted water doesn’t interfere with the function of the secondary limphoid organs.

One of the major aim of the experiment was to correlate the modifications that affect the haematological, biochemical and gravimetric parameters investigated with the level of the plasma deuterium resulted from the deuterium depleted water intake by the animals from the experimental groups. Regarding this aspect (fig. 7), the concentration of the plasma deuterium in groups 2 and 4 was significantly decreased (66.5 ppm), compared to those (groups 1 and 3) which which had received normal water, the deuterium concentration of which was 143 ppm.

In addition to the discussed clinical and haematological results, the tumour volume evaluation revealed that the total quantity of the Ehrlich ascite liquid drawn from the group 3, considered control-tumour group (inoculated only with Ehrlich ascite) was, in the case of all animals, slightly increased than the one obtained in the animals treated with DDW and then inoculated with Ehrlich ascite liquid (tab.1).

Tabel 1. The values of the Ehrlich ascite liquid quantities obtained from the experimental control groups 3 comparative with the group 4 at the end of study.



Group 3

Ehrlich ascite

Group 4

DDW + Ehrlich


4.2 ml (+)

3.4 ml (-)


3.0 ml (+)

2.0 ml (-)


2.8 ml (+)

2.5 ml (-)


3.7 ml (+)

0.8 ml (-)


1.9 ml (+)

1.7 ml (-)

These results could demonstrate that an increase of the deuterium in the animal organism interfere with the proliferative capacity of both normal and neoplastic cells.

Fig. 8. The main consequences of the daily DDW intake on the animal organism


The conclusions that result from the above discussed data analysis refere to the next main clinical, haematological and biochemical aspects:

1)  The daily intake of deuterium depleted water in adult NMRI mice during the whole experimental period (5 weeks) induced a slight decrease of the body growth rate expressed by minimal variations of this parameter.

2)  The modifications of the haematological parameters value, as leukopenia and those of plasma biochemical components (decreases of the total plasma proteins values) are correlated with a significant decrease of relative weight of thymus and spleen in experimental group inoculated with Ehrlich ascite liquid.

3)  The concentration of the plasma deuterium in the animals “treated” with the deuterium depleted water was significantly decreased (66,5 ppm), compared to those which consumed normal water (plasma level=145.5 ppm).

4)  The clinical differences observed between the ascite development in the case of experimental group 3 (control tumour group) and 4 (Ehrlich ascite + deuterium depleted water) were negative, in the favour of the group 4, even if these variations are not very significant. This aspect may be due to the fact that the deuterium depleted water intake was made during a short period of time (only 5 weeks).


1.   ALTERMATT H.J., 1990, “Heavy-water Enhances the Antineoplasic Effect of 5-Fluoro-Uracil and Bleomycin in Nude Mice Bearing Human Carcinoma” International journal of cancer 45:(3) 475-480.

2.   ALTERMATT H.J.,1988, “Heavy-water delays growth of human carcinoma in nude-mice” Cancer 62:(3) 462-466 ,New-York: Wiley-Liss.

3.   KRITCHEVSKY, D, 1960, “Deuterium Isotope Effects in Chemistry and Biology “Annals of the New-York Academy of Science vol. 84, New-York: Academy of Science.

4.  LAMPRECT, J., 1990, “Mitosis Arrested By Deuterium Oxide – light microscopic, immunofluorescence and ultrastructural characterization“ European Journal of Cell Biology 51: (2) 303-312, Wissen-schaftliche Verlag GMBH.

5.  MARCUS I, 2004, „Biologia şi Patologia Animalelor de Laborator”,  Edit. Risoprint, Cluj-Napoca, 85-97.

6.  OLINESCU, A şi colab., 1980: Immune reaction in Ehrlich ascites tumor bearing mice. Arch. Roum. Pathol. Exp. Microbiol., 39, 249-254.

7.  OLINESCU, A şi colab. 1982, „The role of antigenic expression and immunosuppression factors in the development of the immune response against Ehrlich tumor cells. Oncologia, 21, 227-231.

8.  OLINESCU, A şi colab., 1983: Natural cell-mediated cytotoxicity in Ehrlich ascites tumor bearing mice. Neoplasma, 30, 147-152.

9.   SAMLAY si colab., 1993, “Naturally occurring deuterium is essential for the normal growth rate of cell” FEBS Lett.,vol 317, Nr.1,2,1-4.

10. SAMLAY si colab., 1998, ”The biological effect of deuterium-depleted water, a possible new tool in cancer terapy”, Z. Onkol. / J.of Oncol, 30:4.

11. SAMLAY si colab., 2001, “The biological effect of deuterium-depleted water, a possible new tool in cancer therapy”. Anticancer Research, 21:1617-1621.