Механизм гликолитической оксидоредукции, субстратное фосфорилирование, страница 2

                              /                                                                           | 

               СН₂О – Р = О                                                                   С = О              диоксиацетонфосфат                 

                |             \                                                                           |          

               С = О      ОН                                                                     СН₂ОН  

                |

     НО – С – Н                                  альдолаза                                     

                | 

        Н – С – ОН                                                                                      триодофосфат         

                |                                                                                                 изомераза        

        Н – С – ОН     О – Н  

                |              /    

                СН₂О – Р = О                                                                       Н    

                               \                                                                             /          

                                О – Н                                                                  С = О    

             Фосфат – 1,6 – дифосфат                                                             |

                                                                                                    Н – С – ОН 

                                                                                                            |

                                                                                                            СН₂ОРО₃Н₂   

                                                                                                              3 ФГА

Разрыв  связи   происходит  в  результате   ослабления   связи   между  3  и  4   атомами углерода.

Таким  образом, заканчивается   первый  этап  гликолиза, связанный   с  вкладыванием   энергии   в  процесс  активации  субстратов, при   этом   расходуется  2   молекулы     АТФ.

2 – й  этап:

6)         Оидёт  нуклеофильная   атака  карбонильного   углерода

           //                              Н S – Е – НАД                                                                                   

          С – Н                                                                     Н    ОН                                                                

           |                                                                                 \ /

   Н – С – ОН                                                                       С – SЕНАД      

           |                                                                                  |           

          СН₂ОРО₃Н₂                                                       Н – С – ОН

                                                                                              |

                                                                                             СН₂ОРО₃Н₂

                                                                                            ES - комплекс               

                                                             О                                                                                 О                  

                                                           //                                                                                  //     

                                                         С – SЕНАД * Н + Н                  + Фн                        С – О – РО₃Н₂      

                                                          |                                                                                    |        

                                                  Н – С – ОН                            - НSЕНАД * Н + Н     Н – С – ОН         

                                                          |                                                                                    |    

                                                         СН₂ОРО₃Н₂                                                                СН₂ОРО₃Н₂

                                                                                                                                              1,3 – дифосфо-

                                                                                                                                                                                  глицериновая

                                                                                                                                                                                  кислота  

Эта   стадия   гликолитической   оксидоредукции    управляется  НАД  и   НАД * Н + Н. Повышение  НАД   и  снижение  НАД * Н + Н  активирует   данную  реакцию   и   наоборот.   

3 – й  этап (перенос  макроэргической  связи  Ф_н   на  АДФ)

7)            

ОСООН

                     //                                                                       |

     С -  ОРО₃Н₂            фосфоглицераткиназа       Н – С – ОН                

                          |                                    + АДФ                                         |     

                Н – С – ОН                                                             СН₂ОРО₃Н₂ 

                     |                            - АТФ                               3 – фосфоглицериновая   кислота